Labeled tRNA library
The full repertoire of human tRNAs (46 isoacceptors) has been purified and labeled with a fluorescent tag using Anima’s patented technology. Two complete tRNA libraries have been designed: one tagged with an energy donor and one tagged with an energy acceptor. To monitor the translation of any specific protein, we select the “signature pair” of that protein, selected by our tRNA bioinformatics.
This process is patent protected.
Target translation in Fibrosis:
- Steady state synthesis of collagen in fibroblast
- PSM: Lights OFF
- Activation of collagen synthesis in fibroblast
- PSM: Lights ON
Using FRET signals from signature tRNA pairs of the target protein, one tRNA of the pair is labeled with an energy donor and the other with an energy acceptor. We then visualize and monitor the target’s translation.
Anima’s PSM technology can monitor global translation within cells, providing spatial and temporal resolution. Translation of cell surface receptors is monitored on the Endoplasmic Reticulum (ER) or in the cytoplasm; in neurons, translation is monitored at the cell body, in neurites or in axons.
In addition to translation, tRNA transport along dendrites and axons may be monitored. Spatial resolution allows us to study the local translation response to signals at the synapse.
Image: Neuronal cells with transfected tRNA
Single proteins, or proteins belonging to the same family or process, can be monitored by using specific pair(s) of tRNA. Thus, coordinated translation of proteins in response to external signals can be studied. The response of translation to regulators found upstream in biological processes can be studied, such as inhibitors of mTOR or MAPK.
Post-transcription to translation regulatory mechanisms are novel targets that can be targeted with our technology. These include, among others, proteins that interact with the mRNA and its RNA Binding Proteins (RBPs) and proteins that specifically regulate ribosome activity.
Our platform discovers molecules that target these regulatory proteins and pathways. PSM not only identifies drug candidates, but intrinsically helps in elucidating the pathways and mechanisms-of-action of these molecules.