Translation Control Lab
• Analysis Assessment
• Bio Data Analysis
Anima’s Translation Control Lab (TCL) is purpose-built for the discovery of translation control modulators by integrating our proprietary technologies
- An automated customized high content screening environment built around Operetta machines and state of the art robotics
- Our novel PSM biology assays that selectively monitor protein translation
- Proprietary imaging and data analysis software that selects hit compounds
- High performance, big data cloud architecture, running in Amazon AWS that stores millions of images and project data, with cloud servers running the software and custom user interfaces for ongoing, secure access to all project information from any remote location
With its high-performance architecture, a single TCL can be shared by up to 3 projects, screening against different targets.
Discovery projects use dedicated screening machines in the TCL. Bioinformatics, tRNA pair selection and PSM assays are established, validated and optimized for the target protein and its cellular systems. The TCL is then connected to Anima's cloud where the project is assigned its private, secure space, configured with our imaging and big data analysis software.
Experiment design, Analysis Assessment and Bio Data analysis
The Protein Synthesis Monitoring assay uses labeled tRNA pairs that generate FRET signals to monitor ribosome activity in selected cellular system.
PSM assay in a selected cellular system is used to screen a small molecule library of compounds towards the identification of compounds which change the PSM signal.
Implementation of Anima’s data and analysis in Anima’s Virtual Private Network (VPN)
Images captured by Operetta® are automatically uploaded to the Cloud using proprietary plate processing software.
Unbiased scores are generated from more than 90 features extracted from images.
FRET controls, assay controls are located at specific wells in all screens, enabling strict QA algorithms.
PSM design is based on proprietary mathematical algorithms analyzing the relevant RNA code.
PSM Image Creation
All images are captured by Operetta®, and the clean, calculated, PSM images is generated automatically.
State of the art image analysis algorithms extract unique PSM and additional cellular features as a feed for the big data analysis.
QA measures are executed to eliminate outliers wells and cells and to identify false positives.
Big Data Analysis
The analysis utilizes proprietary biological big data algorithms designed for translation control modulators discovery with high level of statistical significance.
Bio Data Center
All biological data and results are kept in a customized industry standard bio data center.
Big Data Visualization
Powerful visual and analytical tools enable us to view the results online from various mathematical and biological angles