Pathway Biology Resolution

Resolve a selected biological pathway in a disease network to reveal its driving activity.

What it means

A Pathway Biology Resolution Workflow establishes how a pathway operates within a disease network.

It identifies which junctions carry the signal, whether the proposed wiring holds in the disease and cellular context, which node moves first, and where crosstalk with adjacent processes occurs.

This reveals the pathway in its true network context, not as an isolated linear diagram.

Input

A defined biological pathway to be resolved.

Pathway of interest: a list of proteins/mediators leading to a disease endpoint
Disease context
Cell type / cellular system
Perturbations

Output

Cell images of DNA, RNA and protein modifications, amount and localization and of biological activities.

A Biology Resolution Data Package containing thousands of Visual Biology Tokens.

Network-accurate pathway activity map in disease context
Ordered sequence of node activations and regulatory events
Crosstalk and interaction points with adjacent pathways

Delivered as a large-scale experimental evidence package for AI reasoning.

What it resolves

Identifying signal-carrying junctions

Discover which junctions are active in a disease network.

Validate pathway wiring

Confirm whether proposed pathway structure holds true in the selected disease and cell type.

Determine activation order

Establish which nodes activate first and how activity propagates through the pathway.

Reveal crosstalk

Identify where the pathway interacts with and is influenced by adjacent biological pathways.

Prioritize with confidence

Generate network-accurate insights to prioritize experiments, targets and therapeutic strategies.

How it works

1. Define

Define the pathway and biological question

The Agent contextualizes the pathway within the disease network and defines what needs to be resolved.

2. Design

Design visual biology experiments

The Agent designs massively parallel Visual Biology experiments across conditions, perturbations and time.

3. Execute

Run on the BioGPU Runtime

Experiments are executed at scale on the BioGPU Runtime, observing cellular activity directly.

4. Generate Tokens

Generate Visual Biology Tokens

Thousands of Visual Biology Tokens are generated - each capturing an observed cellular activity event.

5. Resolve

Resolve pathway behavior in the disease network

The Agent assembles the evidence to resolve pathway behavior, wiring, sequence and crosstalk in disease context.