Visualize mRNA translation
When the selected “signature” pair is used in our screening system, a light pulse (FRET) is generated whenever the pair sits in a ribosome. With Translationlight, it is possible for the first time to visualize protein translation inside living cells. This capability allows Anima to discover compounds that control the process by either increasing or decreasing the translation of a specific mRNA in a selective manner. “Hit compounds” that decrease or increase the light emitted by the ribosomes are selected for further validation and analysis.
Visualize mRNA transcripts
With TranscriptLight, it is possible to visualize individual or multiple mRNA transcripts across their life cycles, in one unique assay. With proprietary algorithms that analyze generated mRNA images, we can follow mRNA localization, stability, splicing, and modifications and identify mRNA small molecule drugs that regulate these biological processes.
mRNA regulation database
Proprietary algorithms to select a tRNA signature pair
Cerebio is Anima's mRNA regulation database and proprietary algorithms that select a tRNA signature pair. Cerebio includes an mRNA in-depth analysis database with mechanisms involved in the regulation of a gene of interest. On top of that, Cerebio runs proprietary algorithms used to select a unique tRNA signature pair that is subsequently transfected into cells during TranslationLight assay. The signature pair is used with high frequency during the target protein's translation but is not frequently used by the background human proteome. 85% of known proteins have a tRNA pair with such a “signal to noise” ratio of at least 20, making them suitable for TranslationLight assay.
Automated high scale mRNA data analysis
All the mRNA images showing the compounds' effect on mRNA biology are automatically captured during screening and instantly uploaded to the cloud for mRNA high scale data analysis by BrightNy. BrightNy selects and validates the active compounds using Anima's proprietary T-Light modeling algorithms that collect dozens of features from the images. The most promising hits are passed on to Compass MOAi.
AI-driven MOA elucidation
Compass is Anima's proprietary technology for the elucidation of compounds' mechanisms of action (MOA) and molecular targets in the novel target space of mRNA biology regulation. Compass is built around Anima's MOAi technology, a breakthrough approach that, for the first time ever, integrates Artificial Intelligence technology for MOA elucidation. MOAi runs along the discovery process:
- AI Image analysis for MOA recognition | Compare images coming from a phenotypic screen to a database of billions of previously seen images and known MOAs and creates a ranked MOA suspects list
- Machine Learning guided MOA assays | Dozens of proprietary MOA assays and experiments, executed in sequence, guided by machine learning technology
Compass rapidly answers the key questions of when, where and how the compounds work along the mRNA life cycle and their mechanisms of action and molecular targets.